Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ROBO1 (Q9Y6N7) - Overview - Molecular Target Synopsis

Protein


ROBO1, Roundabout homolog 1
UniProt Q9Y6N7

Also Known as ROBO1_HUMAN, ROBO1, DUTT1

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development. Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding (PubMed:24673457). Interacts with SLIT1 (By similarity). Interacts with SLIT2 (PubMed:10102268, PubMed:11404413, PubMed:17848514). Interacts with FLRT3 (PubMed:24560577). Interacts with MYO9B (via Rho-GAP domain) (PubMed:26529257).

6A78
CRYSTAL STRUCTURE OF THE FIFTH IMMUNOGLOBULIN DOMAIN (IG5) OF HUMAN ROBO1 IN COMPLEX WITH THE SCFV FRAGMENT OF MURINE MONOCLONAL ANTIBODY B5209B
RCSB/PDB
Inspect Structure
See all 3D Structures for ROBO1

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: ROBO1 is active in the following subcellular-locations: axon, cell membrane, cell projection, endoplasmic reticulum-golgi intermediate compartment membrane.
GO terms: ROBO1 is active in the following subcellular-locations: axon, cell surface, cytoplasm, endoplasmic reticulum-Golgi intermediate compartment membrane, integral component of plasma membrane, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ROBO1 has gain in 3 cell-lines, loss in 7 cell-lines and no signal in 995 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: HS578T, M14, UACC_62

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HCC12, COV434, NCI-H1155

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, SK-N-SH, BJ

(see details)

3D Structures


For ROBO1 there are:
12 structures (15 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis