Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

WNK2 (Q9Y3S1) - Overview - Molecular Target Synopsis

Protein


WNK2, Serine/threonine-protein kinase WNK2
Enzyme Classification 2.7.11.1
UniProt Q9Y3S1

Also Known as WNK2_HUMAN, WNK2, KIAA1760, PRKWNK2, SDCCAG43

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity. Forms a complex with the phosphorylated form of STK39.

6FBK
CRYSTAL STRUCTURE OF THE HUMAN WNK2 CCT-LIKE 1 DOMAIN IN COMPLEX WITH A WNK1 RFXV PEPTIDE
RCSB/PDB
Inspect Structure
See all 3D Structures for WNK2

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: WNK2 is active in the following subcellular-locations: cell membrane, cytoplasm.
GO terms: WNK2 is active in the following subcellular-locations: cytoplasm, cytosol, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project WNK2 has gain in 1 cell-lines, loss in 1 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SN12C, SW_620, RPMI_8226

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H187, SCLC-21H, OVKATE

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

RNA Interference


WNK2 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: HS578T, MDAMB134VI. (see details)

3D Structures


For WNK2 there are:
2 structures (2 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


WNK2 has been screened with 203 compounds (367 bioactivities). (see details)