Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

POLI (Q9UNA4) - Overview - Molecular Target Synopsis

Protein


POLI, DNA polymerase iota
Enzyme Classification 2.7.7.7
UniProt Q9UNA4

Also Known as POLI_HUMAN, POLI, RAD30B

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. Interacts with REV1 (By similarity). Interacts with POLH.

5ULX
STRUCTURE OF HUMAN DNA POLYMERASE IOTA BOUND TO TEMPLATE 1-METHYL- DEOXYADENOSINE CRYSTALLIZED IN THE PRESENCE OF DCTP
RCSB/PDB
Inspect Structure
See all 3D Structures for POLI

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: POLI is active in the following subcellular-locations: nucleus.
GO terms: POLI is active in the following subcellular-locations: cytoplasmic ribonucleoprotein granule, intracellular, nuclear speck, nucleoplasm.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project POLI has gain in 3 cell-lines, loss in 16 cell-lines and no signal in 986 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: T47D, HL_60, MCF7

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: SK-MM-2, NCI-H1930, NCI-H1092

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HMEC, HSMM

(see details)

3D Structures


For POLI there are:
46 structures (48 chains) solved
33 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


POLI has been screened with 4 compounds (4 bioactivities). (see details)