Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
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Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
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Ngly1 (Q9JI78) - Overview - Molecular Target Synopsis

Protein


Ngly1, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Enzyme Classification 3.5.1.52
UniProt Q9JI78

Also Known as NGLY1_MOUSE, Ngly1

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with the proteasome components RAD23B and PSMC1. Interacts with directly with VCP. Interacts with DERL1, bringing it close to the endoplasmic reticulum membrane. Interacts with SAKS1.

2HPL
CRYSTAL STRUCTURE OF THE MOUSE P97/PNGASE COMPLEX
RCSB/PDB
Inspect Structure
See all 3D Structures for Ngly1

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
651Q9JI78-1

Sub-cellular localization


UniProt: Ngly1 is active in the following subcellular-locations: cytoplasm.
GO terms: Ngly1 is active in the following subcellular-locations: collagen-containing extracellular matrix, cytoplasm, cytosol, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project Ngly1 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For Ngly1 there are:
8 structures (9 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis