Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

POMK (Q9H5K3) - Overview - Molecular Target Synopsis

Protein


POMK, Protein O-mannose kinase
Enzyme Classification 2.7.1.183
UniProt Q9H5K3

Also Known as SG196_HUMAN, POMK, SGK196

Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
350ENSG00000185900ENST00000331373ENSP00000331258Q9H5K3-1
134ENSG00000185900ENST00000518991ENSP00000429090

Sub-cellular localization


UniProt: POMK is active in the following subcellular-locations: endoplasmic reticulum membrane.
GO terms: POMK is active in the following subcellular-locations: endoplasmic reticulum membrane, integral component of membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project POMK has gain in 10 cell-lines, loss in 5 cell-lines and no signal in 989 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: ChaGo-K-1, HCC12, CAL-120

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HSMM, HMEC, NHLF

(see details)

RNA Interference


POMK was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: SCC90, VP229. (see details)

3D Structures


At greater than 75% identity similarity to POMK there are:
2 structures (2 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis