Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

NAA50 (Q9GZZ1) - Overview - Molecular Target Synopsis

Protein


NAA50, N-alpha-acetyltransferase 50
Enzyme Classification 2.3.1.258
UniProt Q9GZZ1

Also Known as NAA50_HUMAN, NAA50, MAK3, NAT13, NAT5

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine (PubMed:19744929, PubMed:22311970, PubMed:21900231, PubMed:27484799). Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position (PubMed:27484799). Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins (PubMed:19744929). Autoacetylates in vivo (PubMed:19744929). The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo (PubMed:19744929). Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides (PubMed:16507339, PubMed:27484799). Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10 (PubMed:17502424, PubMed:27422821). Component of a complex composed of NAA50, NAA15 and NAA10 (PubMed:16507339). Interacts with NAA35.

4X5K
HUMAN NAA50 COMPLEX WITH COENZYME A AND AN ACETYLATED PEPTIDE
RCSB/PDB
Inspect Structure
See all 3D Structures for NAA50

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: NAA50 is active in the following subcellular-locations: cytoplasm, nucleus.
GO terms: NAA50 is active in the following subcellular-locations: cytoplasm, cytosol, extracellular exosome, NatA complex, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project NAA50 has gain in 2 cell-lines, loss in 3 cell-lines and no signal in 1000 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: OVCAR433, JIMT-1, KYSE-510

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HMEC, MCF-7

(see details)

3D Structures


For NAA50 there are:
4 structures (10 chains) solved
4 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis