Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

MAP3K14 (Q99558) - Overview - Molecular Target Synopsis

Protein


MAP3K14, Mitogen-activated protein kinase kinase kinase 14
Enzyme Classification 2.7.11.25
UniProt Q99558

Also Known as M3K14_HUMAN, MAP3K14, NIK

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91.

4IDV
CRYSTAL STRUCTURE OF NIK WITH COMPOUND 4-{3-[2-AMINO-5-(2- METHOXYETHOXY)PYRIMIDIN-4-YL]-1H-INDOL-5-YL}-2-METHYLBUT-3-YN-2-OL (13V)
RCSB/PDB
Inspect Structure
See all 3D Structures for MAP3K14

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
947Q99558-1

Sub-cellular localization


UniProt: MAP3K14 is active in the following subcellular-locations: cytoplasm.
GO terms: MAP3K14 is active in the following subcellular-locations: cytoplasm, cytosol, fibrillar center, intracellular membrane-bounded organelle, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project MAP3K14 has gain in 2 cell-lines, loss in 0 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: 786_0, BT_549, DU_145

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HCC1576, NCI-H1299, T98G

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, A549, IMR-90

(see details)

RNA Interference


MAP3K14 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: PCI30, OVTOKO. (see details)

3D Structures


For MAP3K14 there are:
4 structures (12 chains) solved
3 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


MAP3K14 has been screened with 648 compounds (871 bioactivities), 526 compounds have bioactivities that show binding affinity of <= 500nM (683 bioactivities). (see details)