Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

TP53RK (Q96S44) - Overview - Molecular Target Synopsis


TP53RK, EKC/KEOPS complex subunit TP53RK
Enzyme Classification 3.6.-.-
UniProt Q96S44

Also Known as PRPK_HUMAN, TP53RK, C20orf64, PRPK

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806). Component of the EKC/KEOPS complex composed of at least GON7, TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.

Isoforms / Transcripts (Protein Coding)

Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Sub-cellular localization

UniProt: TP53RK is active in the following subcellular-locations: cytoplasm, nucleus.
GO terms: TP53RK is active in the following subcellular-locations: cytoplasm, cytosol, EKC/KEOPS complex, nucleoplasm, nucleus.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project TP53RK has gain in 12 cell-lines, loss in 0 cell-lines and no signal in 993 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: MALME_3M, UACC_62, UACC_257

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HT-144, MKN-45, SK-N-DZ

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HeLa-S3, HSMM, NHLF

(see details)

RNA Interference

TP53RK was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: COV362, OVISE. (see details)

3D Structures

At greater than 35% identity similarity to TP53RK there are:
7 structures (10 chains) solved
6 are solved in complex with at least one small molecule ligand
1 are solved with an approved drug

TP53RK is solved in complex with the approved drug(s):


(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

TP53RK has been screened with 16 compounds (16 bioactivities). (see details)