Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

TP53RK (Q96S44) - Overview - Molecular Target Synopsis

Protein


TP53RK, EKC/KEOPS complex subunit TP53RK
Enzyme Classification 3.6.-.-
UniProt Q96S44

Also Known as PRPK_HUMAN, TP53RK, C20orf64, PRPK

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806). Component of the EKC/KEOPS complex composed of at least GON7, TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
253ENSG00000172315ENST00000372114ENSP00000361186Q96S44-1
121ENSG00000172315ENST00000372102ENSP00000361174

Sub-cellular localization


UniProt: TP53RK is active in the following subcellular-locations: cytoplasm, nucleus.
GO terms: TP53RK is active in the following subcellular-locations: cytoplasm, cytosol, EKC/KEOPS complex, nucleoplasm, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project TP53RK has gain in 12 cell-lines, loss in 0 cell-lines and no signal in 993 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: MALME_3M, UACC_62, UACC_257

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HT-144, MKN-45, SK-N-DZ

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HeLa-S3, HSMM, NHLF

(see details)

RNA Interference


TP53RK was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: COV362, OVISE. (see details)

3D Structures


At greater than 35% identity similarity to TP53RK there are:
7 structures (10 chains) solved
6 are solved in complex with at least one small molecule ligand
1 are solved with an approved drug

TP53RK is solved in complex with the approved drug(s):

AMP/ADENOSINE PHOSPHATE (4WW7_A).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


TP53RK has been screened with 16 compounds (16 bioactivities). (see details)