Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

NEK9 (Q8TD19) - Overview - Molecular Target Synopsis

Protein


NEK9, Serine/threonine-protein kinase Nek9
Enzyme Classification 2.7.11.1
UniProt Q8TD19

Also Known as NEK9_HUMAN, NEK9, KIAA1995, NEK8, NERCC

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. Interacts with DYNLL1; phosphorylation at Ser-944 strongly reduces DYNLL1 binding.

3ZKE
11 RESIDUE FRAGMENT OF
NEK9 PROTEIN
IN THE STRUCTURE OF
STRUCTURE OF LC8 IN COMPLEX WITH NEK9 PEPTIDE
RCSB/PDB
Inspect Structure
See all 3D Structures for NEK9

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: NEK9 is active in the following subcellular-locations: cytoplasm, nucleus.
GO terms: NEK9 is active in the following subcellular-locations: cytosol, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project NEK9 has gain in 6 cell-lines, loss in 0 cell-lines and no signal in 999 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: OVCAR_5, SNB_75, DU_145

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H209, NCI-H1581, HT-144

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HeLa-S3

(see details)

RNA Interference


NEK9 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: LM7, COV362. (see details)

3D Structures


For NEK9 there are:
2 structures (12 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


NEK9 has been screened with 533 compounds (966 bioactivities), 3 compounds have bioactivities that show binding affinity of <= 500nM (3 bioactivities). (see details)