Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ULK2 (Q8IYT8) - Overview - Molecular Target Synopsis

Protein


ULK2, Serine/threonine-protein kinase ULK2
Enzyme Classification 2.7.11.1
UniProt Q8IYT8

Also Known as ULK2_HUMAN, ULK2, KIAA0623

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Interacts with SYNGAP1 (By similarity). Component of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1/FIP200. Interacts (via C-terminus) with ATG13/KIAA0652. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR.

6QAT
CRYSTAL STRUCTURE OF ULK2 IN COMPLEXED WITH HESPERADIN
RCSB/PDB
Inspect Structure
See all 3D Structures for ULK2

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: ULK2 is active in the following subcellular-locations: cytoplasmic vesicle membrane.
GO terms: ULK2 is active in the following subcellular-locations: cytoplasmic vesicle membrane, cytosol, membrane, phagophore assembly site, phagophore assembly site membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ULK2 has gain in 8 cell-lines, loss in 11 cell-lines and no signal in 986 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: HS578T, SK_OV_3, MDA_MB_231

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: MFM-223, SNU-182, NCI-H209

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, BJ, AG445

(see details)

RNA Interference


ULK2 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: SCC90, TE6. (see details)

3D Structures


For ULK2 there are:
3 structures (11 chains) solved
3 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ULK2 has been screened with 266 compounds (435 bioactivities), 20 compounds have bioactivities that show binding affinity of <= 500nM (22 bioactivities). (see details)