Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

NEK8 (Q86SG6) - Overview - Molecular Target Synopsis

Protein


NEK8, Serine/threonine-protein kinase Nek8
Enzyme Classification 2.7.11.1
UniProt Q86SG6

Also Known as NEK8_HUMAN, NEK8, JCK, NEK12A

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway. Interacts with PKD2; may regulate PKD2 targeting to the cilium (By similarity). Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme. Interacts with ANKS3.

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: NEK8 is active in the following subcellular-locations: cell projection, cilium, cytoplasm, cytoskeleton.
GO terms: NEK8 is active in the following subcellular-locations: ciliary base, ciliary inversin compartment, cilium, cytoplasm, cytoskeleton.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project NEK8 has gain in 5 cell-lines, loss in 2 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H441, MOLP-2, OVISE

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, K562, CD20-positive B cell

(see details)

RNA Interference


NEK8 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: CAL120, MIAPACA2. (see details)

3D Structures


At greater than 35% identity similarity to NEK8 there are:
41 structures (47 chains) solved
37 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


NEK8 has been screened with 12 compounds (12 bioactivities). (see details)