Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

NEK8 (Q86SG6) - Overview - Molecular Target Synopsis


NEK8, Serine/threonine-protein kinase Nek8
Enzyme Classification
UniProt Q86SG6

Also Known as NEK8_HUMAN, NEK8, JCK, NEK12A

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway. Interacts with PKD2; may regulate PKD2 targeting to the cilium (By similarity). Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme. Interacts with ANKS3.

Isoforms / Transcripts (Protein Coding)

Sub-cellular localization

UniProt: NEK8 is active in the following subcellular-locations: cell projection, cilium, cytoplasm, cytoskeleton.
GO terms: NEK8 is active in the following subcellular-locations: ciliary base, ciliary inversin compartment, cilium, cytoplasm, cytoskeleton.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project NEK8 has gain in 5 cell-lines, loss in 2 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H441, MOLP-2, OVISE

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, K562, CD20-positive B cell

(see details)

RNA Interference

NEK8 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: CAL120, MIAPACA2. (see details)

3D Structures

At greater than 35% identity similarity to NEK8 there are:
41 structures (47 chains) solved
37 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

NEK8 has been screened with 12 compounds (12 bioactivities). (see details)