Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

TSSK4 (Q6SA08) - Overview - Molecular Target Synopsis


TSSK4, Testis-specific serine/threonine-protein kinase 4
Enzyme Classification
UniProt Q6SA08

Also Known as TSSK4_HUMAN, TSSK4, C14orf20, STK22E, TSSK5

Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (By similarity). May be involved in the Cre/Creb signaling pathway (By similarity). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (PubMed:15964553). Phosphorylates CREM on 'Ser-116' in vitro (By similarity). Phosphorylates ODF2 on 'Ser-95'. Homodimer (By similarity). Interacts with HSP90; this interaction stabilizes and activates TSSK4 (By similarity). Interacts with ODF2 (via C-terminus); this interaction promotes ODF2 phosphorylation on 'Ser-95' (By similarity). May interact with CREM (By similarity). Interacts with CREB1; this interaction facilitates phosphorylation on 'Ser-133' (PubMed:15964553).

Isoforms / Transcripts (Protein Coding)

Sub-cellular localization

UniProt: TSSK4 is active in the following subcellular-locations: acrosome, cell projection, cilium, cytoplasmic vesicle, flagellum, secretory vesicle.
GO terms: TSSK4 is active in the following subcellular-locations: acrosomal vesicle, cytoplasm, motile cilium, nucleus.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project TSSK4 has gain in 2 cell-lines, loss in 2 cell-lines and no signal in 1001 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: HS578T, TK_10, ACHN

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: KYSE-140, Jurkat, Clone E6-1, NCI-H187

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, MCF-7, NHLF

(see details)

RNA Interference

TSSK4 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: CROE33, H358. (see details)

3D Structures

At greater than 35% identity similarity to TSSK4 there are:
17 structures (47 chains) solved
3 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

TSSK4 has been screened with compounds ( bioactivities). (see details)