Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

TSSK4 (Q6SA08) - Overview - Molecular Target Synopsis

Protein


TSSK4, Testis-specific serine/threonine-protein kinase 4
Enzyme Classification 2.7.11.1
UniProt Q6SA08

Also Known as TSSK4_HUMAN, TSSK4, C14orf20, STK22E, TSSK5

Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (By similarity). May be involved in the Cre/Creb signaling pathway (By similarity). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (PubMed:15964553). Phosphorylates CREM on 'Ser-116' in vitro (By similarity). Phosphorylates ODF2 on 'Ser-95'. Homodimer (By similarity). Interacts with HSP90; this interaction stabilizes and activates TSSK4 (By similarity). Interacts with ODF2 (via C-terminus); this interaction promotes ODF2 phosphorylation on 'Ser-95' (By similarity). May interact with CREM (By similarity). Interacts with CREB1; this interaction facilitates phosphorylation on 'Ser-133' (PubMed:15964553).

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: TSSK4 is active in the following subcellular-locations: acrosome, cell projection, cilium, cytoplasmic vesicle, flagellum, secretory vesicle.
GO terms: TSSK4 is active in the following subcellular-locations: acrosomal vesicle, cytoplasm, motile cilium, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project TSSK4 has gain in 2 cell-lines, loss in 2 cell-lines and no signal in 1001 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: HS578T, TK_10, ACHN

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: KYSE-140, Jurkat, Clone E6-1, NCI-H187

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, MCF-7, NHLF

(see details)

RNA Interference


TSSK4 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: CROE33, H358. (see details)

3D Structures


At greater than 35% identity similarity to TSSK4 there are:
17 structures (47 chains) solved
3 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


TSSK4 has been screened with compounds ( bioactivities). (see details)