Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

SPOUT1 (Q5T280) - Overview - Molecular Target Synopsis

Protein


SPOUT1, Putative methyltransferase C9orf114
Enzyme Classification 2.1.1.-
UniProt Q5T280

Also Known as CI114_HUMAN, SPOUT1, C9orf114

Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266). May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (PubMed:28431233). Interacts with INCA1.

4RG1
METHYLTRANSFERASE DOMAIN OF C9ORF114
RCSB/PDB
Inspect Structure
See all 3D Structures for SPOUT1

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
376ENSG00000198917ENST00000361256ENSP00000354812Q5T280-1
87ENSG00000198917ENST00000467582ENSP00000473640

Sub-cellular localization


UniProt: SPOUT1 is active in the following subcellular-locations: centromere, centrosome, chromosome, cytoplasm, cytoskeleton, kinetochore, microtubule organizing center, spindle.
GO terms: SPOUT1 is active in the following subcellular-locations: condensed chromosome kinetochore, cytoplasm, kinetochore, mitotic spindle, spindle pole centrosome.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project SPOUT1 has gain in 0 cell-lines, loss in 1 cell-lines and no signal in 1004 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are:

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are:

(see details)

3D Structures


For SPOUT1 there are:
1 structures (2 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis