Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

MYLK3 (Q32MK0) - Overview - Molecular Target Synopsis

Protein


MYLK3, Myosin light chain kinase 3
Enzyme Classification 2.7.11.18
UniProt Q32MK0

Also Known as MYLK3_HUMAN, MYLK3, MLCK

Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
819ENSG00000140795ENST00000394809ENSP00000378288Q32MK0-1
478ENSG00000140795ENST00000536476ENSP00000439297Q32MK0-4

Sub-cellular localization


UniProt: MYLK3 is active in the following subcellular-locations: cytoplasm.
GO terms: MYLK3 is active in the following subcellular-locations: actin cytoskeleton, cytoplasm, cytosol.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project MYLK3 has gain in 2 cell-lines, loss in 3 cell-lines and no signal in 999 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: WSU-FSCCL, HEp-2, NCI-H2172

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

RNA Interference


MYLK3 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: COV362, HCC70. (see details)

3D Structures


At greater than 50% identity similarity to MYLK3 there are:
1 structures (2 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


MYLK3 has been screened with 81 compounds (81 bioactivities), 13 compounds have bioactivities that show binding affinity of <= 500nM (13 bioactivities). (see details)