Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RAB35 (Q15286) - Overview - Molecular Target Synopsis

Protein


RAB35, Ras-related protein Rab-35
UniProt Q15286

Also Known as RAB35_HUMAN, RAB35, RAB1C, RAY

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes. Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner (PubMed:20154091, PubMed:22065758). Interacts with DENND1C; weak interaction which is nucleotide-independent (PubMed:20154091). Interacts (GTP-bound form) with ACAP2 and MICALL1; the interaction is direct and probably recruits ACAP2 and MICALL1 to membranes (PubMed:21951725). Interacts with EHD1; the interaction is indirect through MICALL1 and probably recruits EHD1 to membranes.

6IF2
COMPLEX STRUCTURE OF RAB35 AND ITS EFFECTOR RUSC2
RCSB/PDB
Inspect Structure
See all 3D Structures for RAB35

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: RAB35 is active in the following subcellular-locations: cell membrane, clathrin-coated pit, clathrin-coated vesicle, cytoplasmic vesicle, endosome, melanosome, membrane.
GO terms: RAB35 is active in the following subcellular-locations: anchored component of synaptic vesicle membrane, cell projection membrane, clathrin-coated endocytic vesicle, clathrin-coated pit, clathrin-coated vesicle membrane, cytosol, endosome membrane, extracellular exosome, Golgi membrane, intercellular bridge, melanosome, plasma membrane, recycling endosome membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RAB35 has gain in 2 cell-lines, loss in 0 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SR, K_562, LOXIMVI

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: SK-N-FI, Hs 746T, NCI-H2804

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, A549, NHLF

(see details)

3D Structures


For RAB35 there are:
4 structures (6 chains) solved
3 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RAB35 has been screened with compounds ( bioactivities). (see details)