Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

MAD2L1 (Q13257) - Overview - Molecular Target Synopsis

Protein


MAD2L1, Mitotic spindle assembly checkpoint protein MAD2A
UniProt Q13257

Also Known as MD2L1_HUMAN, MAD2L1, MAD2

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD (via ubiquitin-like 1 domain) during mitosis. Interacts with isoform 1 and isoform 2 of NEK2. Interacts with HSF1; this interaction occurs in mitosis (PubMed:18794143).

6F0X
CRYO-EM STRUCTURE OF TRIP13 IN COMPLEX WITH ATP GAMMA S, P31COMET, C- MAD2 AND CDC20
RCSB/PDB
Inspect Structure
See all 3D Structures for MAD2L1

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: MAD2L1 is active in the following subcellular-locations: centromere, chromosome, cytoplasm, cytoskeleton, kinetochore, nucleus, spindle pole.
GO terms: MAD2L1 is active in the following subcellular-locations: chromosome, condensed chromosome kinetochore, cytosol, kinetochore, mitotic spindle, nuclear pore nuclear basket, nucleoplasm, nucleus, perinuclear region of cytoplasm, spindle pole.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project MAD2L1 has gain in 0 cell-lines, loss in 5 cell-lines and no signal in 1000 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: HCT_15, MDA_MB_231, HCT_116

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H209, HCC12, NCI-H1155

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HMEC, MCF-7, A549

(see details)

3D Structures


For MAD2L1 there are:
11 structures (42 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis