Molecular Target Synopsis
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RPA49 (Q01080) - Overview - Molecular Target Synopsis

Protein


RPA49, DNA-directed RNA polymerase I subunit RPA49
UniProt Q01080

Also Known as RPA49_YEAST, RPA49, RRN13

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. The heterodimer formed by RPA34 and RPA49 stimulates transcript elongation by Pol I. Subunit RPA49 can bind both single-stranded and double-stranded DNA. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Forms a TFIIF-like heterodimer with RPA34; the heterodimer formed by RPA34 and RPA49 can be dissociated from the Pol I core giving rise to a 12 subunit form A* of Pol I (formerly called pol A) that shows impaired transcript elongation activity and increased sensitivity to alpha-amanitin. The heterodimer formed by RPA34 and RPA49 stabilizes subunit RPA12 and stimulates RPA12-dependent RNA cleavage.

6HKO
YEAST RNA POLYMERASE I ELONGATION COMPLEX BOUND TO NUCLEOTIDE ANALOG GMPCPP
RCSB/PDB
Inspect Structure
See all 3D Structures for RPA49

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
415Q01080-1

Sub-cellular localization


UniProt: RPA49 is active in the following subcellular-locations: nucleolus, nucleus.
GO terms: RPA49 is active in the following subcellular-locations: nucleolus, RNA polymerase I complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RPA49 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For RPA49 there are:
26 structures (32 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis