Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ARF6 (P62330) - Overview - Molecular Target Synopsis

Protein


ARF6, ADP-ribosylation factor 6
UniProt P62330

Also Known as ARF6_HUMAN, ARF6

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes., (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3 (PubMed:11950392). Interacts with PIP5K1C (PubMed:12847086). Interacts with USP6 (via Rab-GAP TBC domain) (PubMed:15509780). Interacts with RAB11FIP3 and RAB11FIP4 (PubMed:16148947, PubMed:17030804, PubMed:17628206). Interacts with HERC1 (PubMed:15642342). Interacts with ARHGAP21 (PubMed:15793564). Interacts with ASAP3; the interaction is stabilized by calcium ions (PubMed:16737952, PubMed:20510928). Interacts with NCS1/FREQ at the plasma membrane (PubMed:17555535). Interacts with TBC1D24 (PubMed:20727515). Interacts with ECPAS (PubMed:20682791). Interacts with MICALL1 (PubMed:21951725). Interacts with SPAG9 homodimers, forming heterotetramers (PubMed:19644450). Interacts with CYTH3 (PubMed:23940353). Interacts with ASAP2 (By similarity). Interacts with UACA (By similarity). Interacts with KIF23, forming heterodimers and heterotetramers (By similarity). Interacts with C9orf72.

6BBP
MODEL FOR COMPACT VOLUME OF TRUNCATED MONOMERIC CYTOHESIN-3 (GRP1; AMINO ACIDS 63-399) E161A 6GS ARF6 Q67L FUSION PROTEIN
RCSB/PDB
Inspect Structure
See all 3D Structures for ARF6

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
335ENSG00000165527ENST00000298316ENSP00000298316
175P62330-1, Q007T5-1, P62332-1, P62331-1

Sub-cellular localization


UniProt: ARF6 is active in the following subcellular-locations: cell membrane, cell projection, cleavage furrow, cytoplasm, cytosol, early endosome membrane, endosome membrane, filopodium membrane, golgi apparatus, midbody, midbody ring, recycling endosome membrane, ruffle, trans-golgi network membrane.
GO terms: ARF6 is active in the following subcellular-locations: cell cortex, cleavage furrow, cytoplasm, cytosol, early endosome membrane, endocytic vesicle, endosome, extracellular exosome, filopodium membrane, Flemming body, focal adhesion, glutamatergic synapse, Golgi apparatus, membrane, plasma membrane, presynapse, recycling endosome membrane, ruffle.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ARF6 has gain in 6 cell-lines, loss in 0 cell-lines and no signal in 999 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: DU_145, TK_10, OVCAR_5

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: G84, DU 145, Hs 746T

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HSMM, HMEC

(see details)

3D Structures


For ARF6 there are:
16 structures (21 chains) solved
14 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ARF6 has been screened with 1 compounds (1 bioactivities). (see details)