Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RAB8A (P61006) - Overview - Molecular Target Synopsis

Protein


RAB8A, Ras-related protein Rab-8A
UniProt P61006

Also Known as RAB8A_HUMAN, RAB8A, MEL, RAB8

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Plays an important role in ciliogenesis (PubMed:21844891). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1, MICALCL, MICAL3 and EHBP1L1 two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1 (By similarity). Interacts with DCDC1 (PubMed:22159412).

5SZI
STRUCTURE OF HUMAN RAB8A IN COMPLEX WITH THE BMERB DOMAIN OF MICAL-CL
RCSB/PDB
Inspect Structure
See all 3D Structures for RAB8A

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
207ENSG00000167461ENST00000300935ENSP00000300935P61007-1, P61006-1, Q4R5P1-1
207ENSG00000167461ENST00000586682ENSP00000467501P61006-2

Sub-cellular localization


UniProt: RAB8A is active in the following subcellular-locations: cell membrane, cell projection, centriole, centrosome, cilium, cilium basal body, cytoplasm, cytoplasmic vesicle, cytoskeleton, golgi apparatus, microtubule organizing center, midbody, phagosome, phagosome membrane, recycling endosome membrane.
GO terms: RAB8A is active in the following subcellular-locations: centriole, centrosome, ciliary basal body, ciliary base, cilium, cytosol, dendritic spine, endosome, extracellular exosome, glutamatergic synapse, Golgi membrane, midbody, neuronal cell body, non-motile cilium, phagocytic vesicle, phagocytic vesicle membrane, plasma membrane, postsynaptic density, recycling endosome membrane, synaptic vesicle, trans-Golgi network membrane, trans-Golgi network transport vesicle.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RAB8A has gain in 2 cell-lines, loss in 1 cell-lines and no signal in 1001 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: HL_60, PC_3, HCT_15

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: Panc 03.27, OCI-LY-3, SNU-398

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HSMM, HMEC

(see details)

3D Structures


For RAB8A there are:
9 structures (24 chains) solved
8 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RAB8A has been screened with compounds ( bioactivities). (see details)