Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

NEK4 (P51957) - Overview - Molecular Target Synopsis


NEK4, Serine/threonine-protein kinase Nek4
Enzyme Classification
UniProt P51957

Also Known as NEK4_HUMAN, NEK4, STK2

Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage. Interacts with RPGRIP1 and RPGRIP1L.

Isoforms / Transcripts (Protein Coding)

Sub-cellular localization

UniProt: NEK4 is active in the following subcellular-locations: cell projection, cilium, cytoplasm.
GO terms: NEK4 is active in the following subcellular-locations: ciliary basal body, ciliary plasm, ciliary rootlet, ciliary transition zone, cytoplasm, cytosol.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project NEK4 has gain in 0 cell-lines, loss in 8 cell-lines and no signal in 997 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: SR, MDA_MB_231, SF_268

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: SU-DHL-1, MKN-45, GP2d

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HeLa-S3, MCF-7

(see details)

RNA Interference

NEK4 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: HELA, BT20. (see details)

3D Structures

At greater than 50% identity similarity to NEK4 there are:
2 structures (4 chains) solved
1 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

NEK4 has been screened with 112 compounds (114 bioactivities), 1 compounds have bioactivities that show binding affinity of <= 500nM (1 bioactivities). (see details)