Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

NEK3 (P51956) - Overview - Molecular Target Synopsis

Protein


NEK3, Serine/threonine-protein kinase Nek3
Enzyme Classification 2.7.11.1
UniProt P51956

Also Known as NEK3_HUMAN, NEK3

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: NEK3 is active in the following subcellular-locations: axon, cell projection, cytoplasm.
GO terms: NEK3 is active in the following subcellular-locations: axon, cytoplasm, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project NEK3 has gain in 2 cell-lines, loss in 4 cell-lines and no signal in 999 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: COLO205, HL_60, HCC_2998

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: TYK-nu, SNU-C1, COLO 206F

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HSMM, A549

(see details)

RNA Interference


NEK3 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: CAOV3, H460. (see details)

3D Structures


At greater than 50% identity similarity to NEK3 there are:
2 structures (4 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


NEK3 has been screened with 247 compounds (418 bioactivities), 1 compounds have bioactivities that show binding affinity of <= 500nM (1 bioactivities). (see details)