Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ARL3 (P36405) - Overview - Molecular Target Synopsis

Protein


ARL3, ADP-ribosylation factor-like protein 3
UniProt P36405

Also Known as ARL3_HUMAN, ARL3, ARFL3

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed:16525022, PubMed:18588884). Required for normal cytokinesis and cilia signaling (PubMed:22085962). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed:30269812). Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed:22085962). Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium. Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B) (PubMed:11303027, PubMed:11847227, PubMed:18588884). Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3 (PubMed:16472755). Interacts with SYS1 (PubMed:11303027, PubMed:11847227, PubMed:15077113). The GTP-bound form interacts with ARL2BP and PDE6D (PubMed:11303027, PubMed:11847227). Microtubule-associated protein (PubMed:11303027, PubMed:16525022). May interact with GOLGA4 (PubMed:11303027). Interacts with GGA1; the interaction recruits PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting of PKD1:PKD2 complex.

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
182ENSG00000138175ENST00000260746ENSP00000260746P36405-1

Sub-cellular localization


UniProt: ARL3 is active in the following subcellular-locations: cell projection, centrosome, cilium, cytoplasm, cytoskeleton, golgi apparatus membrane, microtubule organizing center, nucleus, spindle.
GO terms: ARL3 is active in the following subcellular-locations: centrosome, cilium, cytoplasmic microtubule, extracellular exosome, Golgi apparatus, Golgi membrane, microtubule cytoskeleton, midbody, nucleus, photoreceptor connecting cilium, spindle microtubule.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ARL3 has gain in 1 cell-lines, loss in 1 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: T47D, SN12C, SK_OV_3

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HCC12, OVSAHO, T-47D

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HSMM, IMR-90

(see details)

3D Structures


At greater than 90% identity similarity to ARL3 there are:
6 structures (9 chains) solved
6 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ARL3 has been screened with compounds ( bioactivities). (see details)