Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

ARL3 (P36405) - Overview - Molecular Target Synopsis


ARL3, ADP-ribosylation factor-like protein 3
UniProt P36405

Also Known as ARL3_HUMAN, ARL3, ARFL3

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed:16525022, PubMed:18588884). Required for normal cytokinesis and cilia signaling (PubMed:22085962). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed:30269812). Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed:22085962). Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium. Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B) (PubMed:11303027, PubMed:11847227, PubMed:18588884). Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3 (PubMed:16472755). Interacts with SYS1 (PubMed:11303027, PubMed:11847227, PubMed:15077113). The GTP-bound form interacts with ARL2BP and PDE6D (PubMed:11303027, PubMed:11847227). Microtubule-associated protein (PubMed:11303027, PubMed:16525022). May interact with GOLGA4 (PubMed:11303027). Interacts with GGA1; the interaction recruits PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting of PKD1:PKD2 complex.

Isoforms / Transcripts (Protein Coding)

Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Sub-cellular localization

UniProt: ARL3 is active in the following subcellular-locations: cell projection, centrosome, cilium, cytoplasm, cytoskeleton, golgi apparatus membrane, microtubule organizing center, nucleus, spindle.
GO terms: ARL3 is active in the following subcellular-locations: centrosome, cilium, cytoplasmic microtubule, extracellular exosome, Golgi apparatus, Golgi membrane, microtubule cytoskeleton, midbody, nucleus, photoreceptor connecting cilium, spindle microtubule.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project ARL3 has gain in 1 cell-lines, loss in 1 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: T47D, SN12C, SK_OV_3

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: HCC12, OVSAHO, T-47D

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HSMM, IMR-90

(see details)

3D Structures

At greater than 90% identity similarity to ARL3 there are:
6 structures (9 chains) solved
6 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

ARL3 has been screened with compounds ( bioactivities). (see details)