Molecular Target Synopsis
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RPA12 (P32529) - Overview - Molecular Target Synopsis

Protein


RPA12, DNA-directed RNA polymerase I subunit RPA12
UniProt P32529

Also Known as RPA12_YEAST, RPA12, RRN4

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. Proposed to contribute to the polymerase catalytic activity and form the polymerase active center together with the two largest subunits. Subunit RPA12 contributes a catalytic zinc ribbon that is required for RNA cleavage by Pol I. Involved in transcriptional termination. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Interacts with RPA2/RPA135. Peripheral subunit that binds the catalytic zinc ion that is required for RNA cleavage. The heterodimer formed by RPA34 and RPA49 stabilizes subunit RPA12 and stimulates RPA12-dependent RNA cleavage. Involved in the recruitment of RPA49 to Pol I.

6HLR
YEAST RNA POLYMERASE I ELONGATION COMPLEX BOUND TO NUCLEOTIDE ANALOG GMPCPP (CORE FOCUSED)
RCSB/PDB
Inspect Structure
See all 3D Structures for RPA12

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
125P32529-1

Sub-cellular localization


UniProt: RPA12 is active in the following subcellular-locations: nucleolus, nucleus.
GO terms: RPA12 is active in the following subcellular-locations: RNA polymerase I complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RPA12 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For RPA12 there are:
27 structures (28 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis