Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

PPIF (P30405) - Overview - Molecular Target Synopsis

Protein


PPIF, Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Enzyme Classification 5.2.1.8
UniProt P30405

Also Known as PPIF_HUMAN, PPIF, CYP3

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis. Associates with the mitochondrial membrane ATP synthase F(1)F(0) ATP synthase; the association is increased by inorganic phosphate (Pi) and decreased by cyclosporin A (CsA). Interacts with ATP5F1B; ATP5PD and ATP5PO. Interacts with SLC25A3; the interaction is impaired by CsA. Interacts with BCL2; the interaction is impaired by CsA. Interacts with TP53; the association implicates preferentially tetrameric TP53, is induced by oxidative stress and is impaired by CsA. Interacts with C1QBP. Interacts with MCUR1 (PubMed:26976564). Component of the mitochondrial permeability transition pore complex (mPTPC), at least composed of SPG7, VDAC1 and PPIF (PubMed:26387735). Interacts with SPG7 (PubMed:26387735).

5NWO
HUMAN CYCLOPHILIN D COMPLEXED WITH INHIBITOR
RCSB/PDB
Inspect Structure
See all 3D Structures for PPIF

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: PPIF is active in the following subcellular-locations: mitochondrion matrix.
GO terms: PPIF is active in the following subcellular-locations: membrane, mitochondrial inner membrane, mitochondrial matrix, mitochondrial permeability transition pore complex, mitochondrion.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project PPIF has gain in 8 cell-lines, loss in 1 cell-lines and no signal in 996 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: PC_3, SR, SN12C

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: Hs 746T, HN, HEL

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HeLa-S3, A549

(see details)

3D Structures


For PPIF there are:
41 structures (43 chains) solved
33 are solved in complex with at least one small molecule ligand
1 are solved with an approved drug

PPIF is solved in complex with the approved drug(s):

BS4/SULFABENZAMIDE (3RCF_A).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


PPIF has been screened with compounds ( bioactivities). (see details)