Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

Nos2 (P29477) - Overview - Molecular Target Synopsis

Protein


Nos2, Nitric oxide synthase, inducible
Enzyme Classification 1.14.13.39
UniProt P29477

Also Known as NOS2_MOUSE, Nos2, Inosl

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed:16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8. Homodimer (PubMed:10769116, PubMed:11669619). Interacts with SLC9A3R1 (By similarity). Interacts with GAPDH (By similarity). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (By similarity). Interacts with SPSB1, SPSB2 and SPSB4 (PubMed:20603330). Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4.

4UX6
24 RESIDUE FRAGMENT OF
NITRIC OXIDE SYNTHASE, INDUCIBLE
IN THE STRUCTURE OF
THE DISCOVERY OF NOVEL, POTENT AND HIGHLY SELECTIVE INHIBITORS OF INDUCIBLE NITRIC OXIDE SYNTHASE (INOS)
RCSB/PDB
Inspect Structure
See all 3D Structures for Nos2

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
1144P29477-1

Sub-cellular localization


UniProt: Nos2 is active in the following subcellular-locations: cytoplasm, cytosol.
GO terms: Nos2 is active in the following subcellular-locations: cortical cytoskeleton, cytoplasm, cytosol, extracellular space, nucleus, perinuclear region of cytoplasm, peroxisome, plasma membrane, vesicle membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project Nos2 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For Nos2 there are:
51 structures (91 chains) solved
50 are solved in complex with at least one small molecule ligand
35 are solved with an approved drug

Nos2 is solved in complex with the approved drug(s):

H4B/SAPROPTERIN (1DF1_A, 1DF1_B, 1DWX_A, 1DWX_B, 1JWJ_A, 1JWJ_B, 1M8D_A, 1M8D_B, 1M8E_A, 1M8E_B, 1M8H_A, 1M8H_B, 1M8I_A, 1M8I_B, 1M9T_A, 1M9T_B, 1N2N_A, 1N2N_B, 1NOD_A, 1NOD_B, 1QOM_A, 1QOM_B, 1QW4_A, 1QW4_B, 1QW5_A, 1QW5_B, 1R35_A, 1R35_B, 1VAF_A, 1VAF_B, 2NOD_A, 2NOD_B, 2Y37_A, 2Y37_B, 3DWJ_A, 3DWJ_B, 3E65_A, 3E65_B, 3E67_A, 3E67_B, 3E68_A, 3E68_B, 3E6L_A, 3E6L_B, 3E6N_A, 3E6N_B, 3E6O_A, 3E6O_B, 3E6T_A, 3E6T_B, 3E7I_A, 3E7I_B, 3E7M_A, 3E7M_B, 3EAI_A, 3EAI_B, 3EBD_A, 3EBD_B, 3EBF_A, 3EBF_B, 3NOD_A, 3NOD_B, 3NQS_A, 3NQS_B, 3NW2_A, 3NW2_B, 4JS9_A, 4JS9_B, 4UX6_B),
CLW/CHLORZOXAZONE (1M8D_A, 1M8D_B).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


Nos2 has been screened with 2081 compounds (3363 bioactivities), 83 compounds have bioactivities that show binding affinity of <= 500nM (87 bioactivities). (see details)