Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

tgt (P28720) - Overview - Molecular Target Synopsis

Protein


tgt, Queuine tRNA-ribosyltransferase
Enzyme Classification 2.4.2.29
UniProt P28720

Also Known as TGT_ZYMMO, tgt

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

6H7C
TRNA GUANINE TRANSGLYCOSYLASE H333A MUTANT APO STRUCTURE
RCSB/PDB
Inspect Structure
See all 3D Structures for tgt

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
386P28720-1

Gene Copy Number Variation


In COSMIC - Cell Lines Project tgt has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For tgt there are:
138 structures (148 chains) solved
113 are solved in complex with at least one small molecule ligand
1 are solved with an approved drug

tgt is solved in complex with the approved drug(s):

AMH/TRANEXAMIC ACID (5V3C_A).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


tgt has been screened with 27 compounds (27 bioactivities), 9 compounds have bioactivities that show binding affinity of <= 500nM (9 bioactivities). (see details)