Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ALOX12 (P18054) - Overview - Molecular Target Synopsis

Protein


ALOX12, Arachidonate 12-lipoxygenase, 12S-type
Enzyme Classification 1.13.11.31
UniProt P18054

Also Known as LOX12_HUMAN, ALOX12, 12LO, LOG12

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.

3D3L
THE 2.6 A CRYSTAL STRUCTURE OF THE LIPOXYGENASE DOMAIN OF HUMAN ARACHIDONATE 12-LIPOXYGENASE, 12S-TYPE (CASP TARGET)
RCSB/PDB
Inspect Structure
See all 3D Structures for ALOX12

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
663ENSG00000108839ENST00000251535ENSP00000251535P18054-1
154ENSG00000108839ENST00000480801ENSP00000467033

Sub-cellular localization


UniProt: ALOX12 is active in the following subcellular-locations: cytoplasm, cytosol, membrane.
GO terms: ALOX12 is active in the following subcellular-locations: cytoplasm, cytosol, extracellular exosome, membrane, sarcolemma.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ALOX12 has gain in 2 cell-lines, loss in 4 cell-lines and no signal in 999 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SK_MEL_5, MDA_MB_435, SF_268

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: KYSE-270, MV-4-11, HEL

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

3D Structures


For ALOX12 there are:
1 structures (2 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ALOX12 has been screened with 467 compounds (555 bioactivities), 28 compounds have bioactivities that show binding affinity of <= 500nM (31 bioactivities). (see details)