Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

MIF (P14174) - Overview - Molecular Target Synopsis

Protein


MIF, Macrophage migration inhibitory factor
Enzyme Classification 5.3.2.1
UniProt P14174

Also Known as MIF_HUMAN, MIF, GLIF, MMIF

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Homotrimer. Interacts with CXCR2 extracellular domain (By similarity). Interacts with the CD74 extracellular domain, COPS5 and BNIPL.

5XEJ
CRYSTAL STRUCTURE OF MACROPHAGE MIGRATION INHIBITORY FACTOR BOUND TO MTX
RCSB/PDB
Inspect Structure
See all 3D Structures for MIF

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
115ENSG00000240972ENST00000215754ENSP00000215754Q6DN04-1, P14174-1, Q4R549-1

Sub-cellular localization


UniProt: MIF is active in the following subcellular-locations: cytoplasm, secreted.
GO terms: MIF is active in the following subcellular-locations: cell surface, cytosol, extracellular exosome, extracellular region, extracellular space, ficolin-1-rich granule lumen, nucleoplasm, secretory granule lumen, vesicle.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project MIF has gain in 5 cell-lines, loss in 3 cell-lines and no signal in 997 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: EKVX, HS578T, MCF7

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: KELLY, G96, COLO 699

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: A549, SK-N-SH, HMEC

(see details)

3D Structures


For MIF there are:
88 structures (295 chains) solved
55 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


MIF has been screened with 378 compounds (761 bioactivities), 46 compounds have bioactivities that show binding affinity of <= 500nM (56 bioactivities). (see details)