Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation

HSD3B1 (P14060) - Overview - Molecular Target Synopsis


HSD3B1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Enzyme Classification
UniProt P14060

Also Known as 3BHS1_HUMAN, HSD3B1, 3BH, HSDB3A

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.

Isoforms / Transcripts (Protein Coding)


HSD3B1 is targeted by Approved Drug Trilostane. (see details)

Sub-cellular localization

UniProt: HSD3B1 is active in the following subcellular-locations: endoplasmic reticulum membrane, mitochondrion membrane.
GO terms: HSD3B1 is active in the following subcellular-locations: endoplasmic reticulum membrane, integral component of membrane, mitochondrial inner membrane, mitochondrial intermembrane space, smooth endoplasmic reticulum membrane.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project HSD3B1 has gain in 4 cell-lines, loss in 3 cell-lines and no signal in 997 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: HL_60, RPMI_8226, MCF7

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H226, NCI-H2803, RERF-GC-1B

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

RNA Interference

HSD3B1 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: . (see details)

Screening and Chemistry

HSD3B1 has been screened with 15 compounds (15 bioactivities), 12 compounds have bioactivities that show binding affinity of <= 500nM (12 bioactivities). (see details)