Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

glnA (P12425) - Overview - Molecular Target Synopsis

Protein


glnA, Glutamine synthetase
Enzyme Classification 6.3.1.2
UniProt P12425

Also Known as GLN1A_BACSU, glnA

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism (PubMed:25691471). It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:24158439). Feedback-inhibited GlnA interacts with and regulates the activity of the transcriptional regulator TnrA (PubMed:11719184, PubMed:12139611). During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation (PubMed:11719184, PubMed:12139611, PubMed:25691471). Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity (PubMed:11719184, PubMed:12139611, PubMed:25691471). In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes (PubMed:25691471). Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity. This inhibitory effect is the highest when both glutamine and AMP are present.

4S0R
STRUCTURE OF GS-TNRA COMPLEX
RCSB/PDB
Inspect Structure
See all 3D Structures for glnA

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
444P12425-1

Sub-cellular localization


UniProt: glnA is active in the following subcellular-locations: cytoplasm.
GO terms: glnA is active in the following subcellular-locations: cytoplasm.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project glnA has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For glnA there are:
6 structures (62 chains) solved
5 are solved in complex with at least one small molecule ligand
3 are solved with an approved drug

glnA is solved in complex with the approved drug(s):

GLN/GLUTAMINE (4LNF_A, 4LNF_B, 4LNF_C, 4LNF_D, 4LNF_E, 4LNF_F, 4LNF_G, 4LNF_H, 4LNF_I, 4LNF_J, 4LNF_K, 4LNF_L, 4LNO_A, 4LNO_B, 4LNO_C, 4LNO_D, 4LNO_E, 4LNO_F, 4S0R_A, 4S0R_B, 4S0R_C, 4S0R_D, 4S0R_E, 4S0R_F, 4S0R_G, 4S0R_H, 4S0R_I, 4S0R_J, 4S0R_K, 4S0R_L, 4S0R_M, 4S0R_N).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


glnA has been screened with compounds ( bioactivities). (see details)