Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

glnA (P12425) - Overview - Molecular Target Synopsis


glnA, Glutamine synthetase
Enzyme Classification
UniProt P12425

Also Known as GLN1A_BACSU, glnA

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism (PubMed:25691471). It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:24158439). Feedback-inhibited GlnA interacts with and regulates the activity of the transcriptional regulator TnrA (PubMed:11719184, PubMed:12139611). During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation (PubMed:11719184, PubMed:12139611, PubMed:25691471). Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity (PubMed:11719184, PubMed:12139611, PubMed:25691471). In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes (PubMed:25691471). Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity. This inhibitory effect is the highest when both glutamine and AMP are present.

Inspect Structure
See all 3D Structures for glnA

Isoforms / Transcripts (Protein Coding)

Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Sub-cellular localization

UniProt: glnA is active in the following subcellular-locations: cytoplasm.
GO terms: glnA is active in the following subcellular-locations: cytoplasm.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project glnA has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures

For glnA there are:
6 structures (62 chains) solved
5 are solved in complex with at least one small molecule ligand
3 are solved with an approved drug

glnA is solved in complex with the approved drug(s):

GLN/GLUTAMINE (4LNF_A, 4LNF_B, 4LNF_C, 4LNF_D, 4LNF_E, 4LNF_F, 4LNF_G, 4LNF_H, 4LNF_I, 4LNF_J, 4LNF_K, 4LNF_L, 4LNO_A, 4LNO_B, 4LNO_C, 4LNO_D, 4LNO_E, 4LNO_F, 4S0R_A, 4S0R_B, 4S0R_C, 4S0R_D, 4S0R_E, 4S0R_F, 4S0R_G, 4S0R_H, 4S0R_I, 4S0R_J, 4S0R_K, 4S0R_L, 4S0R_M, 4S0R_N).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

glnA has been screened with compounds ( bioactivities). (see details)