Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RALA (P11233) - Overview - Molecular Target Synopsis

Protein


RALA, Ras-related protein Ral-A
UniProt P11233

Also Known as RALA_HUMAN, RALA, RAL

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (PubMed:19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed:18756269). Interacts (via effector domain) with RALBP1 (PubMed:7673236). Interacts with EXOC2/Sec5 and EXOC8/Exo84; binding to EXOC2 and EXOC8 is mutually exclusive (PubMed:14525976, PubMed:18756269, PubMed:15920473). Interacts with Clostridium exoenzyme C3 (PubMed:16177825, PubMed:15809419). Interacts with RALGPS1 (PubMed:10747847). Interacts with LPAR1 and LPAR2. Interacts with GRK2 in response to LPAR1 activation. RALA and GRK2 binding to LPAR1 is mutually exclusive (PubMed:19306925). Interacts with CDC42.

2A78
CRYSTAL STRUCTURE OF THE C3BOT-RALA COMPLEX REVEALS A NOVEL TYPE OF ACTION OF A BACTERIAL EXOENZYME
RCSB/PDB
Inspect Structure
See all 3D Structures for RALA

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: RALA is active in the following subcellular-locations: cell membrane, cleavage furrow, midbody, midbody ring.
GO terms: RALA is active in the following subcellular-locations: cell surface, cleavage furrow, cytoplasmic vesicle membrane, extracellular exosome, Flemming body, focal adhesion, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RALA has gain in 8 cell-lines, loss in 1 cell-lines and no signal in 996 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: RXF_393, A498, 786_0

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: MKN-7, Hs 746T, SK-CO-1

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, HUVEC, HSMM

(see details)

3D Structures


For RALA there are:
6 structures (9 chains) solved
6 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RALA has been screened with compounds ( bioactivities). (see details)