Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
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Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
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Germline Genetics

RPA190 (P10964) - Overview - Molecular Target Synopsis

Protein


RPA190, DNA-directed RNA polymerase I subunit RPA190
Enzyme Classification 2.7.7.6
UniProt P10964

Also Known as RPA1_YEAST, RPA190, RPA1, RRN1

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. RPA190 and RPA135 both contribute to the polymerase catalytic activity and together form the Pol I active center. In addition, subunit RPA12 contributes a catalytic zinc ribbon that is required for RNA cleavage by Pol I. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA190 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA.

6HLR
YEAST RNA POLYMERASE I ELONGATION COMPLEX BOUND TO NUCLEOTIDE ANALOG GMPCPP (CORE FOCUSED)
RCSB/PDB
Inspect Structure
See all 3D Structures for RPA190

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
1664P10964-1

Sub-cellular localization


UniProt: RPA190 is active in the following subcellular-locations: nucleolus, nucleus.
GO terms: RPA190 is active in the following subcellular-locations: nucleolus, RNA polymerase I complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RPA190 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For RPA190 there are:
27 structures (28 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RPA190 has been screened with compounds ( bioactivities). (see details)