traM, Relaxosome protein TraM
Also Known as
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair. Homotetramer. 2 homotetramers cooperatively bind to DNA although they do not contact each other; cooperativity is achieved by DNA kinking and unwinding (Probable). Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to the F plasmid origin of transfer (oriT) in a site- and sequence-specific manner. Interacts with TraD. Also interacts with TraY.