Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

traM (P10026) - Overview - Molecular Target Synopsis

Protein


traM, Relaxosome protein TraM
UniProt P10026

Also Known as TRAM1_ECOLI, traM

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair. Homotetramer. 2 homotetramers cooperatively bind to DNA although they do not contact each other; cooperativity is achieved by DNA kinking and unwinding (Probable). Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to the F plasmid origin of transfer (oriT) in a site- and sequence-specific manner. Interacts with TraD. Also interacts with TraY.

4QPQ
MECHANISTIC BASIS OF PLASMID-SPECIFIC DNA BINDING OF THE F PLASMID REGULATORY PROTEIN, TRAM
RCSB/PDB
Inspect Structure
See all 3D Structures for traM

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
127P10026-1

Sub-cellular localization


UniProt: traM is active in the following subcellular-locations: cytoplasm.
GO terms: traM is active in the following subcellular-locations: cytoplasm.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project traM has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For traM there are:
5 structures (22 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis