Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

rpoB (P0A8V2) - Overview - Molecular Target Synopsis

Protein


rpoB, DNA-directed RNA polymerase subunit beta
Enzyme Classification 2.7.7.6
UniProt P0A8V2

Also Known as RPOB_ECOLI, rpoB, groN, nitB, rif, ron, stl, stv, tabD

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

6N61
ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME BOUND TO UPSTREAM FORK PROMOTER DNA AND CAPISTRUIN
RCSB/PDB
Inspect Structure
See all 3D Structures for rpoB

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Drugs


rpoB is targeted by Approved Drugs Rifabutin, Rifampin, Rifapentine, Rifaximin. (see details)
Rifabutin
Rifampin
Rifapentine
Rifaximin

Sub-cellular localization


GO terms: rpoB is active in the following subcellular-locations: cytoplasm, cytosol, membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project rpoB has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For rpoB there are:
56 structures (81 chains) solved
12 are solved in complex with at least one small molecule ligand
4 are solved with an approved drug

rpoB is solved in complex with the approved drug(s):

RFP/RIFAMPIN (4KMU_C, 4KMU_H, 5UAC_C, 5UAH_C, 5UAL_C).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


rpoB has been screened with 70 compounds (160 bioactivities), 2 compounds have bioactivities that show binding affinity of <= 500nM (3 bioactivities). (see details)