Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

thyA (P0A884) - Overview - Molecular Target Synopsis

Protein


thyA, Thymidylate synthase
Enzyme Classification 2.1.1.45
UniProt P0A884

Also Known as TYSY_ECOLI, thyA

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product (PubMed:3286637, PubMed:2223754, PubMed:9826509). This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. This protein also binds to its mRNA thus repressing its own translation (PubMed:7708505). Homodimer.

6NNR
HIGH-RESOLUTION STRUCTURE OF WILD-TYPE E. COLI THYMIDYLATE SYNTHASE
RCSB/PDB
Inspect Structure
See all 3D Structures for thyA

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Sub-cellular localization


UniProt: thyA is active in the following subcellular-locations: cytoplasm.
GO terms: thyA is active in the following subcellular-locations: cytosol.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project thyA has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For thyA there are:
65 structures (115 chains) solved
62 are solved in complex with at least one small molecule ligand
4 are solved with an approved drug

thyA is solved in complex with the approved drug(s):

C2F/LEVOMEFOLIC ACID (1TLS_A, 1TLS_B, 1TSN_A),
D16/RALTITREXED (2KCE_A, 2KCE_B),
MTX/METHOTREXATE (1AXW_A, 1AXW_B).

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


thyA has been screened with 153 compounds (237 bioactivities), 10 compounds have bioactivities that show binding affinity of <= 500nM (16 bioactivities). (see details)