Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

PFKM (P08237) - Overview - Molecular Target Synopsis

Protein


PFKM, ATP-dependent 6-phosphofructokinase, muscle type
Enzyme Classification 2.7.1.11
UniProt P08237

Also Known as PFKAM_HUMAN, PFKM, PFKX

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable). Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region).

4OMT
CRYSTAL STRUCTURE OF HUMAN MUSCLE PHOSPHOFRUCTOKINASE (DISSOCIATED HOMODIMER)
RCSB/PDB
Inspect Structure
See all 3D Structures for PFKM

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: PFKM is active in the following subcellular-locations: cytoplasm.
GO terms: PFKM is active in the following subcellular-locations: 6-phosphofructokinase complex, apical plasma membrane, cytosol, nucleus, sperm principal piece.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project PFKM has gain in 6 cell-lines, loss in 0 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: UACC_62, HCT_116, KM12

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: DMS 53, ChaGo-K-1, A4/Fuk

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HeLa-S3

(see details)

RNA Interference


PFKM was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: HUO9, KNS42. (see details)

3D Structures


For PFKM there are:
1 structures (1 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


PFKM has been screened with 1 compounds (1 bioactivities). (see details)