Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
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Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

prfB (P07012) - Overview - Molecular Target Synopsis

Protein


prfB, Peptide chain release factor RF2
UniProt P07012

Also Known as RF2_ECOLI, prfB, supK

Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (PubMed:11118225, PubMed:17932046). Acts as a peptidyl-tRNA hydrolase (PubMed:22857598, PubMed:27934701). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center and releases the ribosome (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Recruited by ArfA to rescue stalled ribosomes in the absence of a normal stop codon (PubMed:22857598, PubMed:22922063, PubMed:25355516). Interacts with the ribosome (PubMed:22857598, PubMed:22922063, PubMed:25355516, PubMed:12511960, PubMed:12511961). Recruited to stalled ribosomes by ArfA, in the presence of truncated mRNA, ArfA influences RF2 conformation so RF2 can hydrolyze the peptidyl-tRNA bond (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875).

6C4H
CONFORMATION OF METHYLATED GGQ IN THE PEPTIDYL TRANSFERASE CENTER DURING TRANSLATION TERMINATION (PTC REGION)
RCSB/PDB
Inspect Structure
See all 3D Structures for prfB

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
365P07012-1, B1XEH8-1, C5A0G3-1

Sub-cellular localization


UniProt: prfB is active in the following subcellular-locations: cytoplasm.
GO terms: prfB is active in the following subcellular-locations: cytosol.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project prfB has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For prfB there are:
5 structures (5 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis