Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RAD3 (P06839) - Overview - Molecular Target Synopsis

Protein


RAD3, General transcription and DNA repair factor IIH helicase subunit XPD
Enzyme Classification 3.6.4.12
UniProt P06839

Also Known as RAD3_YEAST, RAD3, REM1

ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/RAD3 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription. XPD/RAD3 acts by forming a bridge between TFIIK and the core-TFIIH complex. Involved in the maintenance of the fidelity of DNA replication. Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.

6GYM
STRUCTURE OF A YEAST CLOSED COMPLEX WITH DISTORTED DNA (CCDIST)
RCSB/PDB
Inspect Structure
See all 3D Structures for RAD3

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
778P06839-1

Sub-cellular localization


UniProt: RAD3 is active in the following subcellular-locations: nucleus.
GO terms: RAD3 is active in the following subcellular-locations: cytosol, nucleotide-excision repair factor 3 complex, nucleus, transcription factor TFIIH core complex, transcription factor TFIIH holo complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RAD3 has gain in 0 cell-lines, loss in 0 cell-lines and no signal in 0 cell-lines. (see details)

3D Structures


For RAD3 there are:
5 structures (5 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RAD3 has been screened with compounds ( bioactivities). (see details)