Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

POLB (P06746) - Overview - Molecular Target Synopsis

Protein


POLB, DNA polymerase beta
Enzyme Classification 2.7.7.7
UniProt P06746

Also Known as DPOLB_HUMAN, POLB

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Interacts with FAM168A (PubMed:25260657).

6N2R
BINARY COMPLEX CRYSTAL STRUCTURE OF DNA POLYMERASE BETA WITH 5- CARBOXY-DC (5-CAC) AT THE TEMPLATING POSITION
RCSB/PDB
Inspect Structure
See all 3D Structures for POLB

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: POLB is active in the following subcellular-locations: cytoplasm, nucleus.
GO terms: POLB is active in the following subcellular-locations: cytoplasm, microtubule, nucleoplasm, nucleus, protein-containing complex, spindle microtubule.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project POLB has gain in 6 cell-lines, loss in 5 cell-lines and no signal in 994 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: MALME_3M, OVCAR_3, MOLT_4

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H1734, CAL-120, MCAS

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HMEC, NHEK

(see details)

3D Structures


For POLB there are:
355 structures (356 chains) solved
223 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


POLB has been screened with 193 compounds (214 bioactivities), 1 compounds have bioactivities that show binding affinity of <= 500nM (1 bioactivities). (see details)