TNF, Tumor necrosis factor
Also Known as
TNFA_HUMAN, TNF, TNFA, TNFSF2
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation., The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. Homotrimer. Interacts with SPPL2B.
TNF is targeted by Approved Drugs Etanercept, Mirostipen, Infliximab, Adalimumab, Certolizumab Pegol. (see details)
UniProt: TNF is active in the following subcellular-locations: Cell membrane, Membrane, Secreted.
Mutations in Cancer
|Please click the link to see mutations reported in Cancer Gene Census|
|Please click the link to see mutations reported in ICGC|
|According to the Cosmic, TNF has 4 reported mutations in cell lines ....p.P215P (1), p.P215L (1), p.R207* (1), p.P146L (1)|
The mutation data was obtained from the Sanger Institute Catalogue Of Somatic Mutations In Cancer web site, http://www.sanger.ac.uk/cosmic
Bamford et al (2004) The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website. Br J Cancer, 91,355-358.
|Please click the link to see mutations reported in MoKCa|
Gene Copy Number Variation
TNF has an absolute copy number > 7 in 1 cell lines, the highest copy numbers are in cell lines : SUDHL1. (see details)
In the NCI - NCI 60 Reference panel, the highest expressing cell lines are: HCT116, OVCAR8. (see details)
TNF was reported in the following RNAI studies:
AACR Journals - Results of primary druggable genome v2 (DG2) Achilles heel siRNA screen in KMS11 multiple myeloma cells, the highest RNAi cell lines are: KMS11. (see details)
Screening and Chemistry
TNF has been screened with 188 compounds (272 bioactivities), 16 compounds have bioactivities that show binding affinity of <= 500nM (17 bioactivities). (see details)