Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

HRAS (P01112) - Overview - Molecular Target Synopsis

Protein


HRAS, GTPase HRas
UniProt P01112

Also Known as RASH_HUMAN, HRAS, HRAS1

Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151). In its GTP-bound form interacts with PLCE1 (PubMed:11022048). Interacts with TBC1D10C (PubMed:17230191). Interacts with RGL3 (By similarity). Interacts with HSPD1 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Forms a signaling complex with RASGRP1 and DGKZ (PubMed:11257115). Interacts with RASSF5 (PubMed:18596699). Interacts with PDE6D (PubMed:11980706). Interacts with IKZF3 (PubMed:10369681). Interacts with RACK1 (PubMed:14500341). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity). Interacts with RAPGEF2 (PubMed:10608844, PubMed:11598133).

2C5L
STRUCTURE OF PLC EPSILON RAS ASSOCIATION DOMAIN WITH HRAS
RCSB/PDB
Inspect Structure
See all 3D Structures for HRAS

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
170ENSG00000174775ENST00000493230, ENST00000397594, ENST00000417302ENSP00000434023, ENSP00000380722, ENSP00000388246P01112-2

Sub-cellular localization


UniProt: HRAS is active in the following subcellular-locations: cell membrane, cytoplasm, golgi apparatus, golgi apparatus membrane, nucleus, perinuclear region.
GO terms: HRAS is active in the following subcellular-locations: cytoplasm, cytosol, glutamatergic synapse, Golgi apparatus, Golgi membrane, nucleus, perinuclear region of cytoplasm, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project HRAS has gain in 0 cell-lines, loss in 4 cell-lines and no signal in 1000 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SK_OV_3, HT29, NCI_H522

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: MCF10DCIS.com, BHY, CaR-1

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HMEC, A549

(see details)

3D Structures


For HRAS there are:
169 structures (224 chains) solved
161 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


HRAS has been screened with 91 compounds (117 bioactivities), 17 compounds have bioactivities that show binding affinity of <= 500nM (17 bioactivities). (see details)