Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ADA (P00813) - Overview - Molecular Target Synopsis

Protein


ADA, Adenosine deaminase
Enzyme Classification 3.5.4.4
UniProt P00813

Also Known as ADA_HUMAN, ADA, ADA1

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed:8452534, PubMed:16670267). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). Stimulates plasminogen activation (PubMed:15016824). Plays a role in male fertility (PubMed:21919946, PubMed:26166670). Plays a protective role in early postimplantation embryonic development. Interacts with DPP4 (via extracellular domain) (PubMed:10951221, PubMed:14691230, PubMed:7907293, PubMed:8101391, PubMed:15016824). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4 (PubMed:15016824).

3IAR
THE CRYSTAL STRUCTURE OF HUMAN ADENOSINE DEAMINASE
RCSB/PDB
Inspect Structure
See all 3D Structures for ADA

Isoforms / Transcripts (Protein Coding)


Drugs


ADA is targeted by Approved Drugs Pentostatin, Mercaptopurine. (see details)
Pentostatin
Mercaptopurine

Sub-cellular localization


UniProt: ADA is active in the following subcellular-locations: cell junction, cell membrane, cytoplasm, cytoplasmic vesicle lumen, lysosome.
GO terms: ADA is active in the following subcellular-locations: cell junction, cell surface, cytoplasmic vesicle lumen, cytosol, dendrite cytoplasm, external side of plasma membrane, extracellular space, lysosome, membrane, neuronal cell body, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ADA has gain in 14 cell-lines, loss in 0 cell-lines and no signal in 991 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: MOLT_4, CCRF_CEM, COLO205

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: Jurkat, Clone E6-1, LOU-NH91, KARPAS-1106P

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, A549, HeLa-S3

(see details)

3D Structures


For ADA there are:
1 structures (1 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ADA has been screened with 87 compounds (157 bioactivities), 41 compounds have bioactivities that show binding affinity of <= 500nM (78 bioactivities). (see details)