Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

CDK14 (O94921) - Overview - Molecular Target Synopsis

Protein


CDK14, Cyclin-dependent kinase 14
Enzyme Classification 2.7.11.22
UniProt O94921

Also Known as CDK14_HUMAN, CDK14, KIAA0834, PFTK1

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. Found in a complex with LRP6, CCNY and CAPRIN2 during G2/M stage; CAPRIN2 functions as a scaffold for the complex by binding to CCNY via its N terminus and to CDK14 via its C terminus (PubMed:27821587). Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6 (PubMed:20059949, PubMed:19524571). Interacts with CCDN3 and CDKN1A (PubMed:17517622). Interacts with SEPT8 (PubMed:12098780). Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ (PubMed:16775625).

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: CDK14 is active in the following subcellular-locations: cell membrane, cytoplasm, nucleus.
GO terms: CDK14 is active in the following subcellular-locations: cytoplasm, cytoplasmic cyclin-dependent protein kinase holoenzyme complex, cytosol, nucleus, plasma membrane.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project CDK14 has gain in 10 cell-lines, loss in 1 cell-lines and no signal in 994 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are:

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: SUM 52PE, RL, KMS-28BM

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, AG445, hMSC-AT

(see details)

RNA Interference


CDK14 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: LM7, ASPC1. (see details)

3D Structures


At greater than 50% identity similarity to CDK14 there are:
180 structures (250 chains) solved
162 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


CDK14 has been screened with 148 compounds (227 bioactivities), 8 compounds have bioactivities that show binding affinity of <= 500nM (13 bioactivities). (see details)