Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

ALOX12B (O75342) - Overview - Molecular Target Synopsis

Protein


ALOX12B, Arachidonate 12-lipoxygenase, 12R-type
Enzyme Classification 1.13.11.-
UniProt O75342

Also Known as LX12B_HUMAN, ALOX12B

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
701ENSG00000179477ENST00000319144ENSP00000315167O75342-1

Sub-cellular localization


UniProt: ALOX12B is active in the following subcellular-locations: cytoplasm.
GO terms: ALOX12B is active in the following subcellular-locations: cytosol.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project ALOX12B has gain in 0 cell-lines, loss in 7 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: NCI_H322M, MCF7, NCI_H460

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: MS751, IM-95, Hs 746T

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

3D Structures


At greater than 35% identity similarity to ALOX12B there are:
6 structures (11 chains) solved
2 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


ALOX12B has been screened with 25 compounds (25 bioactivities). (see details)