Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

ALOX12B (O75342) - Overview - Molecular Target Synopsis


ALOX12B, Arachidonate 12-lipoxygenase, 12R-type
Enzyme Classification 1.13.11.-
UniProt O75342

Also Known as LX12B_HUMAN, ALOX12B

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.

Isoforms / Transcripts (Protein Coding)

Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform

Sub-cellular localization

UniProt: ALOX12B is active in the following subcellular-locations: cytoplasm.
GO terms: ALOX12B is active in the following subcellular-locations: cytosol.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project ALOX12B has gain in 0 cell-lines, loss in 7 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: NCI_H322M, MCF7, NCI_H460

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: MS751, IM-95, Hs 746T

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, HSMM

(see details)

3D Structures

At greater than 35% identity similarity to ALOX12B there are:
6 structures (11 chains) solved
2 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

ALOX12B has been screened with compounds ( bioactivities). (see details)