Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
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Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

MITF (O75030) - Overview - Molecular Target Synopsis

Protein


MITF, Microphthalmia-associated transcription factor
UniProt O75030

Also Known as MITF_HUMAN, MITF, BHLHE32

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Homodimer or heterodimer; dimerization is mediated via the coiled coil region (PubMed:24631970). Efficient DNA binding requires dimerization with another bHLH protein (PubMed:14975237). Binds DNA in the form of homodimer or heterodimer with either TFE3, TFEB or TFEC (PubMed:15507434). Interacts with KARS (PubMed:14975237). Identified in a complex with HINT1 and CTNNB1 (PubMed:22647378). Interacts with VSX2 (By similarity).

4C7N
CRYSTAL STRUCTURE OF THE SYNTHETIC PEPTIDE IM10 IN COMPLEX WITH THE COILED-COIL REGION OF MITF
RCSB/PDB
Inspect Structure
See all 3D Structures for MITF

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: MITF is active in the following subcellular-locations: nucleus.
GO terms: MITF is active in the following subcellular-locations: nucleoplasm, nucleus, protein-containing complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project MITF has gain in 7 cell-lines, loss in 7 cell-lines and no signal in 991 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: UACC_257, SK_MEL_28, MALME_3M

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: 928 mel, 624 mel, UACC-257

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: NHLF, AG445, BJ

(see details)

3D Structures


For MITF there are:
1 structures (1 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


MITF has been screened with compounds ( bioactivities). (see details)