Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RNASEH1 (O60930) - Overview - Molecular Target Synopsis

Protein


RNASEH1, Ribonuclease H1
Enzyme Classification 3.1.26.4
UniProt O60930

Also Known as RNH1_HUMAN, RNASEH1, RNH1

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224). Monomer.

3BSU
HYBRID-BINDING DOMAIN OF HUMAN RNASE H1 IN COMPLEX WITH 12- MER RNA/DNA
RCSB/PDB
Inspect Structure
See all 3D Structures for RNASEH1

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: RNASEH1 is active in the following subcellular-locations: cytoplasm.
GO terms: RNASEH1 is active in the following subcellular-locations: cytoplasm.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RNASEH1 has gain in 3 cell-lines, loss in 1 cell-lines and no signal in 1001 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: DU_145, SNB_19, HOP_62

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: NCI-H322T, NCI-H520, LN-18

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, NHLF, A549

(see details)

3D Structures


For RNASEH1 there are:
4 structures (20 chains) solved
1 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RNASEH1 has been screened with 16 compounds (22 bioactivities), 2 compounds have bioactivities that show binding affinity of <= 500nM (2 bioactivities). (see details)