Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

BUB1B (O60566) - Overview - Molecular Target Synopsis

Protein


BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Enzyme Classification 2.7.11.1
UniProt O60566

Also Known as BUB1B_HUMAN, BUB1B, BUBR1, MAD3L, SSK1

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with KNL1. Interacts with RIPK3 (PubMed:29883609).

5K6S
10 RESIDUE FRAGMENT OF
BUBR1
IN THE STRUCTURE OF
THE STRUCTURE OF THE PP2A B56 SUBUNIT BUBR1 COMPLEX
RCSB/PDB
Inspect Structure
See all 3D Structures for BUB1B

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: BUB1B is active in the following subcellular-locations: centromere, centrosome, chromosome, cytoplasm, cytoskeleton, kinetochore, microtubule organizing center, nucleus.
GO terms: BUB1B is active in the following subcellular-locations: anaphase-promoting complex, condensed chromosome kinetochore, condensed chromosome outer kinetochore, condensed nuclear chromosome kinetochore, cytoplasm, cytosol, kinetochore, microtubule organizing center, perinuclear region of cytoplasm, spindle.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project BUB1B has gain in 0 cell-lines, loss in 2 cell-lines and no signal in 1003 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: CCRF_CEM, SF_268, HL_60

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: G22, SK-N-DZ, NCI-H209

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: K562, HMEC, SK-N-SH

(see details)

RNA Interference


BUB1B was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: LM7, MCF12A. (see details)

3D Structures


For BUB1B there are:
8 structures (11 chains) solved
0 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


BUB1B has been screened with compounds ( bioactivities). (see details)