Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

CCNT1 (O60563) - Overview - Molecular Target Synopsis

Protein


CCNT1, Cyclin-T1
UniProt O60563

Also Known as CCNT1_HUMAN, CCNT1

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II)., (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin (PubMed:10393184). Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs (PubMed:17643375). Interacts with BRD4, targets chromatin binding (PubMed:16109376, PubMed:16109377, PubMed:24360279). Interacts with JMJD6 (PubMed:24360279). Interacts with MDFIC (PubMed:12944466). Interacts with HSF1 (PubMed:27189267). Interacts with HTATSF1 (PubMed:10913173). Interacts with AFF4 (PubMed:12065898). Interacts with TBX21 (PubMed:27292648).

6CYT
HIV-1 TAR LOOP IN COMPLEX WITH TAT:AFF4:P-TEFB
RCSB/PDB
Inspect Structure
See all 3D Structures for CCNT1

Isoforms / Transcripts (Protein Coding)


Protein Length Ensembl Gene Ensembl Transcript Ensembl Protein Uniprot Isoform
726ENSG00000129315ENST00000261900ENSP00000261900O60563-1
184ENSG00000129315ENST00000417344ENSP00000399845O60563-2

Sub-cellular localization


UniProt: CCNT1 is active in the following subcellular-locations: nucleus.
GO terms: CCNT1 is active in the following subcellular-locations: cyclin/CDK positive transcription elongation factor complex, nucleoplasm, nucleus.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project CCNT1 has gain in 5 cell-lines, loss in 0 cell-lines and no signal in 1000 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: MOLT_4, HCT_116, OVCAR_8

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: ChaGo-K-1, A4/Fuk, DMS 53

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HeLa-S3, HSMM, NHLF

(see details)

3D Structures


For CCNT1 there are:
24 structures (32 chains) solved
2 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


CCNT1 has been screened with 1229 compounds (1566 bioactivities), 599 compounds have bioactivities that show binding affinity of <= 500nM (640 bioactivities). (see details)