Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
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Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
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RNMT (O43148) - Overview - Molecular Target Synopsis

Protein


RNMT, mRNA cap guanine-N7 methyltransferase
Enzyme Classification 2.1.1.56
UniProt O43148

Also Known as MCES_HUMAN, RNMT, KIAA0398

Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed:9790902, PubMed:9705270, PubMed:10347220, PubMed:11114884, PubMed:22099306, PubMed:27422871). Binds RNA containing 5'-terminal GpppC (PubMed:11114884). Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity (PubMed:11114884). Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates (PubMed:11114884). Interacts with elongating form of polymerase II and RNGTT (PubMed:9705270). Interacts with RAMAC, this interaction significantly enhances RNA-binding and cap methyltransferase activity (PubMed:22099306, Ref.14).

5E8J
CRYSTAL STRUCTURE OF MRNA CAP GUANINE-N7 METHYLTRANSFERASE IN COMPLEX WITH RAM
RCSB/PDB
Inspect Structure
See all 3D Structures for RNMT

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: RNMT is active in the following subcellular-locations: nucleus.
GO terms: RNMT is active in the following subcellular-locations: fibrillar center, mRNA cap binding complex, mRNA cap methyltransferase complex, nucleoplasm, nucleus, receptor complex.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RNMT has gain in 3 cell-lines, loss in 4 cell-lines and no signal in 998 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: SK_MEL_5, MDA_N, SK_MEL_28

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: SK-N-FI, MCAS, CAL-120

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: SK-N-SH, HSMM, K562

(see details)

3D Structures


For RNMT there are:
5 structures (14 chains) solved
5 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis