Molecular Target Synopsis
Overview
Domains and Structures
Drugs and Clinical Candidates
Druggability
Chemistry
Ligand Efficiency Plot
Pathways
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
RNAi
Mutations
Germline Genetics

RIOK3 (O14730) - Overview - Molecular Target Synopsis

Protein


RIOK3, Serine/threonine-protein kinase RIO3
Enzyme Classification 2.7.11.1
UniProt O14730

Also Known as RIOK3_HUMAN, RIOK3, SUDD

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843). Interacts with CASP10 (PubMed:19557502). Interacts with IRF3; RIOK3 probybly mediates the interaction of TBK1 with IRF3 (PubMed:24807708). Associated with 40S pre-ribosomal particles.

Isoforms / Transcripts (Protein Coding)


Sub-cellular localization


UniProt: RIOK3 is active in the following subcellular-locations: cytoplasm.
GO terms: RIOK3 is active in the following subcellular-locations: cytosol, preribosome.



UniProt
GO terms

Gene Copy Number Variation


In COSMIC - Cell Lines Project RIOK3 has gain in 2 cell-lines, loss in 8 cell-lines and no signal in 994 cell-lines. (see details)

Gene Expression


In NCI60, the highest expressing cell lines are: K_562, HS578T, RXF_393

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: OV-90, PA-TU-8988S, NCI-H209

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HSMM, NHLF, SK-N-SH

(see details)

RNA Interference


RIOK3 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: MCF12A, PL5. (see details)

3D Structures


At greater than 35% identity similarity to RIOK3 there are:
7 structures (14 chains) solved
6 are solved in complex with at least one small molecule ligand



(see details)
Molecular Target 3D Synopsis

Screening and Chemistry


RIOK3 has been screened with 102 compounds (140 bioactivities), 8 compounds have bioactivities that show binding affinity of <= 500nM (12 bioactivities). (see details)