Molecular Target Synopsis
Domains and Structures
Drugs and Clinical Candidates
Ligand Efficiency Plot
Family Cladogram
Interaction Network
Gene Expression
Gene Copy Number Variation
Germline Genetics

RIOK3 (O14730) - Overview - Molecular Target Synopsis


RIOK3, Serine/threonine-protein kinase RIO3
Enzyme Classification
UniProt O14730

Also Known as RIOK3_HUMAN, RIOK3, SUDD

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843). Interacts with CASP10 (PubMed:19557502). Interacts with IRF3; RIOK3 probybly mediates the interaction of TBK1 with IRF3 (PubMed:24807708). Associated with 40S pre-ribosomal particles.

Isoforms / Transcripts (Protein Coding)

Sub-cellular localization

UniProt: RIOK3 is active in the following subcellular-locations: cytoplasm.
GO terms: RIOK3 is active in the following subcellular-locations: cytosol, preribosome.

GO terms

Gene Copy Number Variation

In COSMIC - Cell Lines Project RIOK3 has gain in 2 cell-lines, loss in 8 cell-lines and no signal in 994 cell-lines. (see details)

Gene Expression

In NCI60, the highest expressing cell lines are: K_562, HS578T, RXF_393

In Array Express (RNA-seq of 675 commonly used human cancer cell lines), the highest expressing cell lines are: OV-90, PA-TU-8988S, NCI-H209

In Array Express (RNA-seq of long poly adenylated RNA and long non poly adenylated RNA from ENCODE cell lines), the highest expressing cell lines are: HSMM, NHLF, SK-N-SH

(see details)

RNA Interference

RIOK3 was reported in the following RNAI studies:

Cell - Large Scale Profiling of Kinase Dependencies in Cancer Cell Lines, the highest RNAi cell lines are: MCF12A, PL5. (see details)

3D Structures

At greater than 35% identity similarity to RIOK3 there are:
7 structures (14 chains) solved
6 are solved in complex with at least one small molecule ligand

(see details)
Molecular Target 3D Synopsis

Screening and Chemistry

RIOK3 has been screened with 102 compounds (140 bioactivities), 8 compounds have bioactivities that show binding affinity of <= 500nM (12 bioactivities). (see details)